Ingestion of microplastic debris by green sea turtles ( Chelonia mydas ) in the Great Barrier Reef: Validation of a sequential extraction protocol (2025)

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Caron, A G.M.; Thomas, C R.; Berry, K L.E.; Motti, C A.; Ariel, E; Brodie, J E.

Marine Pollution Bulletin 127: 743-751

2018

ISSN/ISBN: 0025-326X DOI:10.1016/j.marpolbul.2017.12.062
Accession:065110928
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Ingestion of microplastic debris by green sea turtles ( Chelonia mydas ) in the Great Barrier Reef: Validation of a sequential extraction protocol (1)

Ingestion of microplastic debris by green sea turtles ( Chelonia mydas ) in the Great Barrier Reef: Validation of a sequential extraction protocol (2)


Summary
Ocean contamination by plastics is a global issue. Although ingestion of plastic debris by sea turtles has been widely documented, contamination by microplastics (<5mm) is poorly known and likely to be under-reported. We developed a microplastic extraction protocol for examining green turtle (Chelonia mydas) chyme, which is multifarious in nature, by modifying and combining pre-established methods used to separate microplastics from organic matter and sediments. This protocol consists of visual inspection, nitric acid digestion, emulsification of residual fat, density separation, and chemical identification by Fourier transform infrared spectroscopy. This protocol enables the extraction of polyethylene, high-density polyethylene, (aminoethyl) polystyrene, polypropylene, and polyvinyl chloride microplastics >100μm. Two macroplastics and seven microplastics (two plastic paint chips and five synthetic fabric particles) were isolated from subsamples of two green turtles. Our results highlight the need for more research towards understanding the impact of microplastics on these threatened marine reptiles.

Related References

Caron, A.G.M.; Thomas, C.R.; Berry, K.L.E.; Motti, C.A.; Ariel, E.; Brodie, J.E. 2018: Ingestion of microplastic debris by green sea turtles (Chelonia mydas) in the Great Barrier Reef: Validation of a sequential extraction protocol Marine Pollution Bulletin 127: 743-751

Tucker, A.D.; Read, M.A. 2001: Frequency of foraging by gravid green turtles (Chelonia mydas) at Raine Island, Great Barrier Reef Journal of Herpetology 35(3): 0-3

Ahasan, M.S.; Picard, J.; Elliott, L.; Kinobe, R.; Owens, L.; Ariel, E. 2017: Evidence of antibiotic resistance in Enterobacteriales isolated from green sea turtles, Chelonia mydas on the Great Barrier Reef Marine Pollution Bulletin 120(1-2): 18-27

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Hof, C.; Smallwood, E.; Meager, J.; Bell, I. 2017: First citizen-science population abundance and growth rate estimates for green sea turtles Chelonia mydas foraging in the northern Great Barrier Reef, Australia Marine Ecology Progress Series 574: 181-191

Booth, D., T.; Astill, K. 2001: Temperature variation within and between nests of the green sea turtle, Chelonia mydas (Chelonia: Cheloniidae) on Heron Island, Great Barrier Reef Australian Journal of Zoology 49(1): 71-84

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Greenham, P. 1969: Nesting behavior of the green sea turtle chelonia mydas on a great barrier reef island Herpetologica: 93-102

Limpus, C.J. 1993: The green turtle, Chelonia mydas, in Queensland: Breeding males in the southern Great Barrier Reef Wildlife Research 20(4): 513-523

Wilkinson, A.; Ariel, E.; van de Merwe, J.; Brodie, J. 2023: Green Turtle (Chelonia mydas) Blood and Scute Trace Element Concentrations in the Northern Great Barrier Reef Environmental Toxicology and Chemistry 42(11): 2375-2388

Limpus, C., J.; Miller, J., D.; Parmenter, C.J.; Limpus, D., J. 2003: The green turtle, Chelonia mydas, population of Raine Island and the northern Great Barrier Reef: 1843-2001 Memoirs of the Queensland Museum 49(1): 349-440

Bell, I.P.; Meager, J.; van de Merwe, J.P.; Madden Hof, C.A. 2019: Green turtle (Chelonia mydas) population demographics at three chemically distinct foraging areas in the northern Great Barrier Reef Science of the Total Environment 652: 1040-1050

Scott, A.L.; York, P.H.; Rasheed, M.A. 2020: Green turtle (Chelonia mydas) grazing plot formation creates structural changes in a multi-species Great Barrier Reef seagrass meadow Marine Environmental Research 162: 105183

Booth, D., T.; Freeman, C. 2006: Sand and nest temperatures and an estimate of hatchling sex ratio from the Heron Island green turtle (Chelonia mydas) rookery, Southern Great Barrier Reef Coral Reefs 25(4): 629-633

Sinaei, M.; Zare, R.; Talebi Matin, M.; Ghasemzadeh, J. 2021: Marine Debris and Trace Metal (Cu, Cd, Pb, and Zn) Pollution in the Stranded Green Sea Turtles (Chelonia mydas) Archives of Environmental Contamination and Toxicology 80(3): 634-644

Choi, D.Y.; Gredzens, C.; Shaver, D.J. 2021: Plastic ingestion by green turtles (Chelonia mydas) over 33years along the coast of Texas, USA Marine Pollution Bulletin 173(Part B): 113111

Fuentes, M., M.P.B.; Lawler, I., R.; Gyuris, E. 2006: Dietary preferences of juvenile green turtles (Chelonia mydas) on a tropical reef flat Wildlife Research 33(8): 671-678

Morais, R.A.; Longo, G.O.; Santos, R.A.; Yoshida, E.T.E.; Stahelin, G.D.; Horta, P.A. 2012: Cephalopod ingestion by juvenile green sea turtles chelonia mydas predatory or scavenging behavior Herpetological Review 43(1): 47-50

Petry, M.V.; Araújo, L.D.; Brum, A.ôn.C.; Benemann, V.ór.R.F.; Finger, J.úl.V.ór.G. 2021: Plastic ingestion by juvenile green turtles (Chelonia mydas) off the coast of Southern Brazil Marine Pollution Bulletin 167: 112337


Ingestion of microplastic debris by green sea turtles ( Chelonia mydas ) in the Great Barrier Reef: Validation of a sequential extraction protocol (2025)
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